Holly Willoughby and Phillip Schofield were doing a segment about Britain’s first ever guide ponies on This Morning when the incident occurred.
The presenters were chatting to a woman, called Katy, who uses a therapy horse to help her after she broke her back a few years ago.
However, as Phil asked: “A guide dog is unlikely to poo on the kitchen floor, can they be horse trained?” the pony decided to do some droppings live on the chatshow.
Holly became hysterical over the scenes and started crying with laughter while her co-host joked: “Oh there we go, perfect timing, absolute perfect timing.”
This Morning: The guide pony started pooing LIVE on set [ITV]
This Morning: Holly Willoughby was left in TEARS over the pooing guide pony [ITV]
In fact, Holly was laughing so much that her mascara started running and she was unable to continue presenting.
While Phil tried to keep a straight face in between stifled laughing as he called over crew to clean up the mess.
Show watchers were also left hysterical over the scenes and took to Twitter to share their thoughts.
One person said: “The camera man zooming in on the poo just to make sure everyone saw it.. #ThisMorning.”
This Morning: The guide pony pooed everywhere [ITV]
This Morning: Holly Willoughby and Phillip Schofield were left SPEECHLESS over the poo incident [ITV]
A different account put: “Omg a pony just did a big poo on #ThisMorning I’m howling” followed by crying laughing emojis.
Another user howled: “I can’t stop laughing at @thismorning having their Blue Peter ‘Pony Poo’ moment! #PonyPoo #GuidePony #ThisMorning.”
“What a stunt queen Just as the owner says the ‘guide horse’ can be house trained it does a poo hahaha #ThisMorning,” a different viewer commented.
Another person joked: “HAHAHAHAAHAHAHAHAHAHAHAHAHAH THE HORSE JUST DID A POO ON THE FLOOR OH MY GOSH #ThisMorning.”
This Morning: Holly Willoughby’s mascara even started running [ITV]
While a different user added: “Crying laughing at the little pony having a poo on the carpet. Holly and Philip are hilarious #ThisMorning.”
This Morning continues weekdays at 12:30pm on ITV.