Tristan "snuggled up" with Jordyn Woods at a house party on Sunday – spelling the end of the relationship, according to TMZ.
Jordyn has been a close friend of the Kardashian-Jenner family for years and has even modelled for Khloe's Good American clothing line.
The site explained: "Witnesses tell us Tristan and Jordyn were all over each other making out. We're told Khloe found out and immediately broke things off with the NBA player."
They said a source had revealed: "She has had enough", adding: "Khloe and Tristan are done."
Tristan tweeted that the reports are "FAKE NEWS" before swiftly deleting the post.
The pair's relationship was first rocked in April last year, when Tristan was accused of cheating on Khloe just days before the blonde beauty gave birth.
Khloe allowed her partner to be in the delivery room to welcome baby True in spite of the reports, and the couple ended up remaining together.
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