The reality TV pair are aiming to get £50,000 for Balls to Cancer by losing their locks.
Dan has previously admitted how fond he is of his hair and how he thinks it's his sexiest feature.
Nathan posed gamely with his shorn hair and later posted a picture on Instagram with a fun filter.
The shaving took place at the Refinery Barbers in Mayfair, west London.
Dozens of fans also turned up to watch the big event.
As part of the campaign, Hairburst have pledged £10,000 in addition to the £50,000 target.
Meanwhile Dan, 28, recently got back together with girlfriend Amber Turner.
In between takes for the final scenes of TOWIE the pair looked loved up to the max.
Amber was seen licking his face and an amorous Dan was spotted giving her bum a little squeeze.
Earlier this month Nathan's missus Cara De La Hoyde revealed that she wants another baby already.
Their tot Freddie-George is not yet six-months-old.
On a recent episode of Towie, Cara declared that she’d love to have another baby, if Nathan would allow it.
However, she then jokingly added that they would have to lift their sex ban, with Nathan declaring they were going through a dry spell.
Nathan was one of the original cast members of the 2016 series of Love Island, entering the villa on the first day and staying right through till the end.
He paired up with Cara De La Hoyde on the first episode and their relationship was solid as a rock until the end.
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