The Electoral Commission is expected to find Vote Leave guilty of breaching campaign rules on spending and co-ordinating with other groups.
The pro-Brexit group denies the claims and says it wasn't given the chance to defend itself.
But EU supporters are trying to use the finding as an excuse to hold a second referendum – claiming it makes the first vote invalid.
Tony Blair's spin doctor Alastair Campbell said today: "When there are proven breaches of law in Parliamentary elections you have a by-election."
Labour MP Mike Gapes added: "This is big. Vote Leave got the narrow less than 4% win by overspending and breaking the law.
"Lying and Cheating. Even more reason for Parliament to take back control."
And Eloise Todd, head of the anti-Brexit campaign Best for Britain which is funded by George Soros, said: "It's good to see the Electoral Commission are taking action to help protect our democratic process, but they can't undo the consequences of Vote Leave's actions.
"Today’s revelations lay bare the need for the public to have the final say on the Brexit deal, in a campaign very different to the last."
The Electoral Commission is set to conclude that Vote Leave wrongly declared £250,000 in spending on the 2016 referendum campaign.
The watchdog will also say the group has failed to provide all the receipts required to check its spending.
And it will criticise campaigners for co-ordinating their efforts with other pro-Brexit groups, which is against the rules.
But Vote Leave chief Matthew Elliott has vowed to challenge the Electoral Commission in the courts.
He also says the watchdog refused to interview anyone from the group as part of its investigation.
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