The happy couple are set to tie the knot at St George’s Chapel with a regal escort including Prince George and Princess Charlotte.
The Queen's great-granddaughter, 28, will say her wedding vows with Eton-educated Tequila ambassador Tom at exactly 12 noon on Friday.
The couple have invited 850 guests to their special day – 250 more than Meghan Markle and Prince Harry.
Several members of the Royal Family are set to attend, including Prince William, Kate Middleton, Prince Harry and Meghan Markle.
Prince Charles, however, will attend his niece’s wedding alone as Camilla has a previously-arranged royal engagement.
Celebrity guests will include singer Robbie Williams and wife Ayda Field.
The Grenadier Guards, of which the bride’s father, Prince Andrew is colonel, will line the West Steps as the couple leave as man and wife. They will then parade through the streets of Windsor in an open-top carriage.
Prince George, five, and his little sister Princess Charlotte, three, are back in the wedding spotlight as a page boy and bridesmaid.
They will be joined by Zara and Mike Tindall’s daughter Mia, four, and Peter and Autumn Phillips’ daughters Savannah, seven, and six-year-old Isla.
During the service, which takes place at 11am, opera star Andrea Bocelli, 60, will sing two songs while state trumpeters from the Band of the Household Cavalry will perform a fanfare as the bride arrives.
Eugenie’s goddaughter, Maud Windsor, the five-year-old granddaughter of Princess Michael of Kent, also makes the party and the second pageboy is Louis de Givenchy, six, the son of high-flying JP Morgan banker Olivier and his wife Zoe who are close friends.
Eugenie, 28, has also created special “attendant” roles for her cousins Louise, 14, and James, ten, the children of the Earl and Countess of Wessex.
The wedding service will include a personal prayer written by Archbishop of York John Sentamu.
Dean of Windsor David Conner will give the address and before officiating as the couple make their marriage vows.
After the ceremony, guests will enjoy a traditional champagne reception hosted by the Queen.
The evening reception is being organised by wedding planner Peregrine Armstrong-Jones, the man behind David and Victoria Beckham's 1999 big day.
They will have a sit-down meal at the Royal Lodge, where Princess Eugenie grew up and Prince Andrew and mum Sarah Ferguson still live – despite being divorced for 22 years.
The couple have opted for a lavish three-course dinner in a private marquee laid with a glass dance floor.
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